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Amelioration of progressive renal injury by genetic manipulation of Klotho gene

Statistical analysis In the in vitro alkaline comet assay, significant differences between control and treated cells were evaluated using the unpaired Welchs t -test, one-tailed (PCLiS, PHH and HepG2 cells), as equal variance was not assumed, or paired t -test, one-tailed (PRH, due to variability of each rat), using TIBCOs Statistica (StatSoft GmbH, Germany)

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The transfection assay clearly demonstrated a decrease in glutamine consumption due to reduced GLS1 levels, whereas this was not as apparent in the dendriplex PURE G4 -LA 12 -anti- GLS1 -siRNA assay

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